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Plos One : Characterization of a New Series of Fluorescent Probes for Imaging Membrane Order, Volume 7

By Johannes, Ludger

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Book Id: WPLBN0003934921
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Characterization of a New Series of Fluorescent Probes for Imaging Membrane Order, Volume 7  
Author: Johannes, Ludger
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Publication Date:
Publisher: Plos

Description : Visualization and quantification of lipid order is an important tool in membrane biophysics and cell biology, but the availability of environmentally sensitive fluorescent membrane probes is limited. Here, we present the characterization of the novel fluorescent dyes PY3304, PY3174 and PY3184, whose fluorescence properties are sensitive to membrane lipid order. In artificial bilayers, the fluorescence emission spectra are red-shifted between the liquid-ordered and liquiddisordered phases. Using ratiometric imaging we demonstrate that the degree of membrane order can be quantitatively determined in artificial liposomes as well as live cells and intact, live zebrafish embryos. Finally, we show that the fluorescence lifetime of the dyes is also dependent on bilayer order. These probes expand the current palate of lipid ordersensing fluorophores affording greater flexibility in the excitation/emission wavelengths and possibly new opportunities in membrane biology.


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